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KMID : 0895820100200020017
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Journal of Oriental Rehabilitation Medicine
2010 Volume.20 No. 2 p.17 ~ p.34
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Effects of Gwanjul8-bang on Collagen Induced Arthritis in DBA/1J Mice
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Kim Jong-Kook
Oh Min-Seok
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Abstract
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Objectives : This study was carried out to know the effects of Gwanjul8-bang (here in after reffered to GJ8) on the inhibition of arthritis induced by collagen on DBA/1J mice.
Methods : For this purpose, GJ8 was orally administered to mouse with arthritis induced by collagen Ⅱ. Cytotoxicity, hepatotoxicity, arthritis index, value of immunocyte in draining lymph node(DLN) and paw joint, cytokine in serum were measured in vivo.
Results : 1. The arthritis index was decreased significantly. 2. In total cell counts of DLN and paw joint, the cells in DLN increased significantly while there was a significant decrease in paw joint. 3. In lymph nodes, CD19+, CD3+, CD4+/CD25+ cells increased significantly, and B220+/CD23+ cells decreased significantly. 4. In joints, CD3+, CD4+ and CD11b+/Gr-1+ cells decreased significantly. 5. The levels of TNF-α, IL-6, IL-17, MCP-1 and vascular endothelial growth factor(VEGF) in serum was significantly decreased compared with control. 6. Anti-collagen Ⅱ in serum was significantly decreased compared with control. 7. The degree of arthritis induced damage of joint of GJ8 group is slight compared with control group in histopathologic observation(hematoxylin & eosin(H&E), Masson-Trichrome(MT) staining).
Conclusions : Comparison of the results for this study showed that GJ8 had immunomodulatory effects. So we expect that GJ8 should be used as a effective drugs for not only rheumatoid arthritis but also another auto-immune disease. Therefore there seems to be many clinical research needed in the future.
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KEYWORD
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Gwanjul8-bang(GJ8), Rheumatoid arthritis, DBA/1J mouse, Collagen induced arthritis, Immunocyte
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